Research Article |
Corresponding author: Antonio Giacò ( antonio.giaco@biologia.unipi.it ) Academic editor: Gianniantonio Domina
© 2024 Antonio Giacò, Angelino Carta, Jacopo Franzoni, Alessio Mo, Mario Pentassuglia, Lorenzo Peruzzi.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Giacò A, Carta A, Franzoni J, Mo A, Pentassuglia M, Peruzzi L (2024) Chromosome numbers for the Italian flora: 14. Italian Botanist 17: 69-75. https://doi.org/10.3897/italianbotanist.17.128997
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In this contribution, new chromosome data obtained on material collected in Italy are presented. It includes the first counts for four subspecies of the Italian endemic Centaurea aplolepa Moretti, i.e. C. aplolepa subsp. aplolepa, C. aplolepa subsp. bertolonii, C. aplolepa subsp. levantina, and C. aplolepa subsp. parvula. In addition, the first chromosome count for an Italian population of Silene canescens (Caryophyllaceae) is provided.
Asteraceae, Caryophyllaceae, Centaurea, cytotaxonomy, endemism, Silene
Texts concerning new chromosome data should be submitted electronically to Antonio Giacò (antonio.giaco@biologia.unipi.it), including indications on voucher specimens and methods used.
Centaurea aplolepa subsp. aplolepa (Asteraceae)
Chromosome number. 2n = 18 (Fig.
Voucher specimen. Italy. Liguria. Capo Noli (Savona), sulle pareti rocciose (WGS84: 44.196751°N, 8.424978°E), 12 July 2023, A. Giacò, L. Peruzzi (PI066169).
Method. Squash preparations were made on root tips obtained from germinating seeds. Root tips were pre-treated with 0.4% colchicine for 3 h and then fixed in Carnoy solution for 1 h. After hydrolysis in 1N HCl at 60 °C for 7.5 minutes, the tips were stained with leuco-basic fuchsine.
Observations. Centaurea aplolepa is endemic to the Tyrrhenian portion of central-northern Italy and includes ten subspecies (
A. Giacò, L. Peruzzi
Centaurea aplolepa subsp. bertolonii (Arrigoni) Greuter (Asteraceae)
Chromosome number. 2n = 18 (Fig.
Voucher specimen. Italy. Liguria. Italia, Genova, nei pressi di Forte Sperone (WGS84: 44.437325°N, 8.931887°E), 12 July 2023, A. Giacò, L. Peruzzi (PI066182).
Method. Squash preparations were made on root tips obtained from germinating seeds. Root tips were pre-treated with 0.4% colchicine for 3 h and then fixed in Carnoy solution for 1 h. After hydrolysis in 1N HCl at 60 °C for 7.5 minutes, the tips were stained with leuco-basic fuchsine.
Observations. Centaurea aplolepa subsp. bertolonii is endemic to the hills over and west of Genova, showing a distribution range that is approximatively in-between those of C. aplolepa subsp. aplolepa to the west and C. aplolepa subsp. levantina and C. aplolepa subsp. lunensis to the east (
A. Giacò, M. Pentassuglia, L. Peruzzi
Centaurea aplolepa subsp. levantina (Arrigoni) Greuter (Asteraceae)
Chromosome number. 2n = 18 (Fig.
Voucher specimen. Italy. Liguria. Sestri Levante (Genova) (WGS84: 44.269873°N, 9.38648°E), piccoli affioramenti rocciosi presso il molo, A. Giacò, L. Peruzzi (PI066170).
Method. Squash preparations were made on root tips obtained from germinating seeds. Root tips were pre-treated with 0.4% colchicine for 3 h and then fixed in Carnoy solution for 1 h. After hydrolysis in 1N HCl at 60 °C for 7.5 minutes, the tips were stained with leuco-basic fuchsine.
Observations. According to
A. Giacò, M. Pentassuglia, L. Peruzzi
Centaurea aplolepa subsp. parvula (Ces.) Arcang. (Asteraceae)
Chromosome number. 2n = 18 (Fig.
Voucher specimen. Italy. Piemonte. Acqui Terme, su calanchi (WGS84: 44.58492°N, 8.35745°E), 12 August 2023, A. Mo (PI066166).
Method. Squash preparations were made on root tips obtained from germinating seeds. Root tips were pre-treated with 0.4% colchicine for 3 h and then fixed in Carnoy solution for 1 h. After hydrolysis in 1N HCl at 60 °C for 7.5 minutes, the tips were stained with leuco-basic fuchsine.
Observations. Albeit Centaurea aplolepa subsp. parvula was not included by
A. Giacò, A. Mo
Silene canescens Ten. (Caryophyllaceae)
Chromosome number. 2n = 24 (Fig.
Voucher specimen. Italy. Toscana. Marina di Torre del Lago (Viareggio, Lucca), retroduna (WGS84: 43.829054°N, 10.253978°E), 15 Jun 2023, A. Mo (seeds collected and deposited at the germplasm bank of the Department of Biology, University of Pisa; a herbarium specimen collected from the same area in 2012 is conserved at PI under the barcode PI063832).
Method. Squash preparations were made on root tips obtained from germinating seeds. Root tips were pre-treated with 0.4% colchicine for 3 h and then fixed in Carnoy solution for 1 h. After hydrolysis in 1 N HCl at 60 °C for 8 minutes, the tips were stained with leuco-basic fuchsine.
Observations. Silene canescens Ten. is a psammophyte belonging to a species complex distributed throughout the sandy shores of southern Europe (
J. Franzoni, A. Carta, A. Mo
AG is thankful to G. Casazza for having provided precise locations of occurrence of Centaurea populations in Liguria.